General Information

  • ID:  hor006185
  • Uniprot ID:  P30253
  • Protein name:  Paralytic peptide 1
  • Gene name:  NA
  • Organism:  Manduca sexta (Tobacco hawkmoth) (Tobacco hornworm)
  • Family:  GBP/PSP1/paralytic peptide family
  • Source:  animal
  • Expression:  Hemolymph.
  • Disease:  NA
  • Comments:  NA
  • Taxonomy:   Manduca (genus), Sphingini (tribe), Sphinginae (subfamily), Sphingidae (family), Bombycoidea (superfamily), Obtectomera , Ditrysia , Heteroneura (parvorder), Neolepidoptera (infraorder), Glossata (suborder), Lepidoptera (order), Amphiesmenoptera (superorder), Endopterygota (cohort), Neoptera (infraclass), Pterygota (subclass), Dicondylia , Insecta (class), Hexapoda (subphylum), Pancrustacea , Mandibulata , Arthropoda (phylum), Panarthropoda , Ecdysozoa , Protostomia , Bilateria , Eumetazoa , Metazoa (kingdom), Opisthokonta , Eukaryota (superkingdom),cellular organisms
  • GO MF:  GO:0005125 cytokine activity
  • GO BP:  GO:0007165 signal transduction
  • GO CC:  GO:0005615 extracellular space

Sequence Information

  • Sequence:  ENFAGGCATGYLRTADGRCKPTF
  • Length:  23
  • Propeptide:  ENFAGGCATGYLRTADGRCKPTF
  • Signal peptide:  NA
  • Modification:  NA
  • Glycosylation:  NA
  • Mutagenesis:  NA

Activity

  • Function:  Causes rapid, rigid paralysis when injected into Lepidopteran larvae. The physiological role may be to reduce hemolymph loss following injury and promote wound healing.
  • Mechanism:  NA
  • Cross BBB:  NA
  • Target:  NA
  • Target Unid:  NA
  • IC50: NA
  • EC50: NA
  • ED50: NA
  • kd: NA
  • Half life: NA

Structure

  • Disulfide bond:  7-19
  • Structure ID:  AF-P30253-F1(AlphaFold_DB_ID)/1HRL(PDB_ID)

  • Structure: (PDB_ID-from https://www.rcsb.org/; AlphaFold_DB_ID-from https://alphafold.ebi.ac.uk/; hordbxxxxxx_AF2.pdb was predicted structure by AlphaFold2; hordbxxxxxx_ESM.pdb was predicted structure by ESMFold)
    •    AF-P30253-F1.pdb1hrl.pdbhor006185_AF2.pdbhor006185_ESM.pdb

Physical Information

Mass: 282831 Formula: C104H159N31O33S2
Absent amino acids: HIMQSVW Common amino acids: G
pI: 8.22 Basic residues: 3
Polar residues: 11 Hydrophobic residues: 6
Hydrophobicity: -44.35 Boman Index: -4278
Half-Life: 1 hour Half-Life Yeast: 30 min
Half-Life E.Coli: >10 hour Aliphatic Index 30
Instability Index: 778.26 Extinction Coefficient cystines: 1615
Absorbance 280nm: 73.41

Literature

  • PubMed ID:  2071576
  • Title:  Isolation and identification of paralytic peptides from hemolymph of the lepidopteran insects Manduca sexta, Spodoptera exigua, and Heliothis virescens.
  • PubMed ID:  10517164
  • Title:  Structure of a paralytic peptide from an insect, Manduca sexta.